Expression Plasmids with Engineered Guide gRNA To Target Any Specific dsDNA Section. Cas9 Enzyme Cuts dsDNA at Target Site For Nucleotide(s) Deletion, Insertion, and Repair.
https://sites.tufts.edu/crispr/genome-editing/
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PCR - DNA Massive Copying Tool
Copying Sample DNA Segment. Polymerase. Two Different Designed Primers. One Forward Primer and Another Reverse Primer - Starters For Polymerases To Build/Extend Polymer Chains. Denature-Anneal-Extension Cycle. ~ 35-60 Repeat Cycles For 10^6 Copies. Amplifying by Massive Copying For Pathogen Detection.
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